Golgi-Cox Impregnation and Staining Service

Bioenno provides Golgi-cox impregnation and staining of dendrites and dendritic spines of neurons found in various parts of the brain including the cortex, hippocampus, and cerebellum of rats, mice, cats, rabbits, and monkeys, as well as tissues obtained from post-mortem human brains. Bioenno will prepare slides and conduct multiple measurements to ensure statistically accurate results.

The measurements include:

  • Sampling: Neurons will be chosen by using systematic unbiased sampling from interested brain areas.
  • Sholl analysis of dendritic branching, including total dendritic length, number of intersections as well as branch points at concentric circles at increasing distance from the soma.
  • Spine analysis, including the number and types of spines (mushroom-type, thin, stubby, and filopodia-like).  Because the density/type of spines is variable from branch to branch, the analysis will be performed by comparing dendritic branches of the same order in all experimental groups.
  • Analysis of soma: the largest area and size of impregnated neuron.

Final Report
Upon completion of the work, Bioenno’s expert team will prepare a comprehensive final report with microscopic images, original measurements, data analysis including statistical analysis and graphis, discussion, per customer reporting requirements.

Importance of Golgi-Cox Impregnation and Staining
Golgi-Cox impregnation and staining allows scientists to clearly visualize the soma, dendrites, and dendritic spines of neurons from the brain tissues of various animals including rats, mice, cats, rabbits, and monkeys, as well as tissues obtained from post-mortem human brains. Understanding the morphology of these cellular structures is critical for research on the effects of various diseases on the brain including Alzheimer’s, Parkinson’s, depression, and stroke. In addition, understanding these structures allows scientists to better understand the effects of aging and the effects of man-made toxins and illicit drugs (e.g. methamphetamine) on the brain. Furthermore, Golgi-Cox impregnation and staining allows scientists to further understand neuroplasticity, neuroprotection, and ultimately how the brain can recover from neuron damage.

Importance of Dendrites of Neurons
Dendrites of neurons make up 95% of the total volume of the neuron. Synapses, which are structures that permit neurons to pass electrical and chemical signals to another cell, are found on dendritic spines. If there are any changes to the dendritic spines, this will cause neuron and potential brain damage. Scientists characterize these dendrites and dendritic spines in order to determine if there is any loss of structure that may lead to neuron and potential brain damage.

 

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