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Bioenno is currently marketing the Benzidine Dihydrochloride (BDHC) Peroxidase Substrate Kit (Catalog Number: 003850). BDHC is a very sensitive chromogen and can produce a blue and particulate reaction product in the presence of peroxidase (HRP) enzyme under acidic conditions. The Bioenno BDHC Substrate Kit contains the reagents necessary to prepare an acidic buffer (pH 6.2 ± 0.1) (1,800 ml) and a BDHC substrate working solution (280 ml). The kit is ideal for dual-labeling immunocytochemistry and immunohistochemistry and can be used together with 3,3’-diaminobenzidine (DAB), the most popularly used chromogen. The blue/particulate reaction product of BDHC is easily distinguished from the brown/diffuse reaction product of DAB (see images and references below). The kit has been tested on tissue sections and cells derived from mice and rats. The BDHC substrate is also suitable for electron microscopy (EM).
Product Description and Protocol
Bioenno Tech has successfully used the BDHC Substrate Kit and DAB Substrate Kit for both single- and double-labeling immunohistochemistry:
Figure: Standard avidin-biotin-peroxidase immunohistochemistry was performed on free-floating sections. BDHC and DAB Substrate Kits were used in the single- and double-labeling
(A) The Bioenno BDHC Substrate Kit was employed, and the boxed area was magnified (100×) to highlight the BDHC-labeled neurons. (B,C) The BDHC Kit was used together with the DAB Kit to present single- and/or dual-labeled neurons. DAB (brown) was used as the first chromogen and BDHC (blue) was the second one. The boxed areas were magnified (63×) to highlight the immuno-labeled neurons. The images were taken from adult rat brains.
Warranty: 12 months from the date of purchase.
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