Immunocytochemistry (ICC) and In Situ Hybridization (ISH) Service
Bioenno is committed to providing high quality and reliable services of immunocytochemistry (ICC) and nonradioactive in situ hybridization (ISH) staining in various parts of the brain of rats, mice, cats, and rabbits. Combining our neuroanatomy expertise with advanced hybridization and immunolabeling techniques, Bioenno will perform single-, double-, or triple-labeling ICC/ISH. The report signals will be visualized by using either chromogenic substrates or fluorescent dyes. Upon completion of the work, Bioenno will prepare a comprehensive final report containing materials, methods, microscopic images, data analysis, and original slides, per customer reporting requirements.
Fig. 1 Bioenno-performed double-labeling immunocytochemistry (ICC).
Chromogens consisted of 3,3′-diaminobenzidine (DAB) (brown) and benzidine dihydrochloride (dark blue). The images were taken from the somatosensory cortex of an adult rat. The boxed area in the top panel (A), shown at 20x objective, was magnified (B, 63x) to highlight the dual-labeled (arrows) and single-labeled (arrowhead) neurons.
Fig. 2 Bioenno-performed double-labeling ICC to present single-labeled neurons (solid and empty arrowheads) and dual-labeled neurons (arrows) in different brain areas.
Chromogens are 3,3′-diaminobenzidine (brown) and benzidine dihydrochloride (dark blue). The images were taken at 63x objective.
Fig. 3 Bioenno-performed nonradioactive in situ hybridization (ISH, blue) and immunocytochemistry (ICC, brown).
Digoxigenin-labeled probe was used and the mRNA hybridization signals were detected by chromogens 4-nitro blue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). The immunoreactive signals were visualized with 3,3′-diaminobenzidine (DAB). The empty arrowheads denote mRNA-expressing neurons, and solid arrowheads indicate protein-expressing neurons. The image was taken from the hippocampus of a young rat at 20x objective.
Fig. 4 Bioenno-performed combined ISH and ICC.
The nonradioactive hybridization signals (mRNA signals) were detected by NBT/BCIP (blue). The immunoreactive signals (protein signals) were visualized with DAB (brown). The image was taken from the accumbens nucleus of an adult rat at 63x objective.
Fig. 5 Bioenno-performed ISH and ICC.
The mRNA hybridization signals were detected by NBT/BCIP (blue). The protein immunoreactive signals were visualized with DAB (brown). The images were taken from the cingulate cortex of an adult rat at 20x (top) and 63x (bottom) objectives.