DAB-Co Substr. Kit (Cat#003843)
DAB-Co Substrate Kit (Catalog Number: 003843)
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Bioenno is currently selling the DAB-Co Substrate Kit (Catalog: 003843). The Bioenno DAB-Cobalt Substrate Kit is an advanced and enhanced version of the DAB (3,3’-diaminobenzidine) Substrate Kit. This kit is ideal for single and/or double labeling immunohistochemistry and immunocytochemistry and can be used on either tissue sections or cells. The incorporation of cobalt into DAB can modify the color of the normally brown DAB reaction, leading to a distinct dark blue/bluish black color. The dark blue DAB-Cobalt reaction product is stable and can be easily distinguished from the brown DAB reaction product. The kit contains all of the necessary reagents to prepare about 330 ml of substrate working solution, and the stock solutions are contained in convenient dropper bottles. The kit can be stored in a dark area at 2-8ºC and is stable for 12 months.
Production Description and Protocol
Bioenno Tech has successfully used the DAB-Co Substrate Kit for single- and double-labeling immunohistochemistry and immunocytochemistry.
Figure: Immunohistochemistry using the Bioenno DAB-Cobalt Substrate Kit
Standard avidin-biotin-peroxidase immunohistochemistry was performed on free-floating rat brain sections. (A) The substrate working solution contains both DAB and Cobalt, and the reaction product is dark blue. (B) Cobalt was not added into the substrate working solution. (C,D) Double-labeling immunohistochemistry performed on free-floating brain sections: A brown reaction product was developed with the substrate solution containing DAB, and dark blue product was developed with the solution containing both DAB and Cobalt. These immunoreaction products can be distinguished on the same brain sections. DAB was used as the first chromogen and DAB-Cobalt was the second one. The boxed areas were magnified (63x) to highlight the DAB and DAB-Cobalt labeled neurons.
Warranty: 12 months from the date of purchase.
Return Policy: Bioenno Tech’s return policy for this product is 90 days from the date of purchase.
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